Dipeptidyl aminopeptidase processing and biosynthesis of alkaline extracellular protease from Yarrowia lipolytica
نویسندگان
چکیده
منابع مشابه
A novel location for dipeptidyl aminopeptidase processing sites in the alkaline extracellular protease of Yarrowia lipolytica.
A stretch of 10 consecutive dipeptides with the sequence -X-Ala- or -X-Pro-, possible cleavage sites for dipeptidyl aminopeptidase (DPAPase) activity, are located in the prepro-region of the alkaline extracellular protease (AEP) beginning at Leu14. Evidence for DPAPase processing of this dipeptide stretch was obtained by characterizing the polypeptide secreted by a strain carrying a xpr6 mutati...
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XPR2 and AXP1, coding for alkaline (AEP) and acid (AXP) extracellular proteases, have been sequenced for several strains. For XPR2, the three sequenced strains are not closely related and produce significantly different levels of AEP, yet the coding sequences are identical, and there is only a single nucleotide difference in one promoter suggesting that host physiology, not promoter differences...
متن کاملCharacterization of an Extracellular lipase from Yarrowia lipolytica
Lipases from different sources have distinct properties, such as specificity, stability and optimal operational conditions. Moreover, different conditions of lipase production may alter these enzyme properties. Therefore, the aims of this study were related to the evaluation of lipase activity of Yarrowia lipolytica IMUFRJ 50682 (isolated from Baía de Guanabara, Brazil) produced under submerged...
متن کاملpH-regulated expression of the acid and alkaline extracellular proteases of Yarrowia lipolytica.
The pH-regulated expression of the acid (AXP) and alkaline (AEP) extracellular proteases of the yeast Yarrowia lipolytica 148 was analysed. Expression in batch and continuous cultures was determined at the mRNA level by Northern blotting, and at the enzyme level by enzyme assays and Western blotting. Culture pH regulated AEP and AXP expression predominantly at the level of mRNA content. Highest...
متن کاملGenetic control of extracellular protease synthesis in the yeast Yarrowia lipolytica.
Depending on the pH of the growth medium, the yeast Yarrowia lipolytica secretes an acidic protease or an alkaline protease, the synthesis of which is also controlled by carbon, nitrogen, and sulfur availability, as well as by the presence of extracellular proteins. Previous results have indicated that the alkaline protease response to pH was dependent on YlRim101p, YlRim8p/YlPalF, and YlRim21p...
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ژورنال
عنوان ژورنال: Microbiology
سال: 1997
ISSN: 1350-0872,1465-2080
DOI: 10.1099/00221287-143-10-3263